The growing interest in green infrastructure places a high expectation on urban plants to make cities and towns more livable now and in the future. Ensuring that plants can survive and grow to their full potential in the face of poor soil conditions, pollution, excessive heat, and sub-optimal water availability is increasingly necessary to improve urban greening. Yet we still have a limited understanding of the ways plants might respond to the many different stressors they encounter in urban landscapes. Plant ecophysiology provides useful frameworks and tools for assessing complex plant responses to varied urban stressors. For instance, fine scale measurements of leaf level gas exchange can be used to evaluate responses to local conditions such as transplant shock and drought, whereas plant water relations theory can be used to estimate plant transpiration at the city scale. Knowledge gained through studying plant ecophysiology in the urban environment can inform both urban landscape planning and management practices, particularly under changing climatic and environmental conditions. Ecophysiological approaches can be further used to improve species selection and create low maintenance/low input urban plantings and landscapes. This session will showcase current urban plant physiology research that bridges theoretical and applied ecology in order to create more sustainable and resilient urban landscapes.